(R)-BPO-27

MCE 国际站：(R)-BPO-27

产品活性：(R)-BPO-27 是 BPO-27 的 R 型对映体，是一种有效的、具有口服活性的、ATP 竞争性的 CFTR 抑制剂，具有 IC₅₀ 的 4 nM。

研究领域：Membrane Transporter/Ion Channel | Autophagy

In Vitro: (R)-BPO-27 exhibits a dose-response inhibition and inhibits the CFTR current by 50% at 0.53 nM in HEK-293T cells. (R)-BPO-27 acts from the cytoplasmic side and has low membrane permeability.(R)-BPO-27 reduces the channel open probability (NPo) from 0.29 to 0.08, modestly reduces in mean channel open time, and strongly increases mean channel closed time in HEK-293T cells expressing human wild-type CFT in a single-channel patch-clamp experiment. Meanwhile, (S)-BPO-27 does not affect any of these parameters.(R)-BPO-27 is applied directly to the cytoplasmic membrane surface and stabilizes the CFTR channel closed state with an IC₅₀ of 600 pM in Single-channel electrophysiology assay.(R)-BPO-27 (10 μM, 10 min pretreatment) inhibits Cl^- current with apparent IC₅₀ values of 5 and 10 nM for CPT-cAMP and 8-Br-cGMP, respectively, in CFTR-expressing FRT cells after CFTR stimulation by cAMP agonist. the IC₅₀ of 4 nM for inhibition of forskolin-stimulated CFTR Cl^- current in FRT cells.

In Vivo: (R)-BPO-27 (interperitoneal administration; 10 mg/kg) decays with t_1/2≈1.6 h and gives sustained therapeutic concentrations in kidney in a PK study.(R)-BPO-27 (intraperitoneal injection; 5 mg/kg; 30 min before abdominal surgery) prevents fluid accumulation in closed midjejunal loops produced by cholera toxin, giving an intestinal loop weight/length ratio similar to that in PBS-injected loops. This effect is dose-dependently and the IC₅₀ value is 0.1 mg/kg.(R)-BPO-27 (intraperitoneal injection or oral administration; 5 mg/kg) shows a slow (R)-BPO-27 metabolism and produces sustained serum (R)-BPO-27 levels for at least 4 h. The AUC analysis gave an oral bioavailability of ∼94% for (R)-BPO-27 in mouse pharmacokinetics and toxicity study.

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