ACSL4 polyclonal antibody
产品名称： ACSL4 polyclonal antibody
英文名称： ACSL4 polyclonal antibody
产品编号： PAB2504
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ACSL4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 244 -274 at internal region of human ACSL4.
- Sequence:
- IHSMQSVEELGSNPENLGIPPSRPTPSDMAI
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- ACSL3 and GSK-3?] are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells.
Chang YS, Tsai CT, Huangfu CA, Huang WY, Lei HY, Lin CF, Su IJ, Chang WT, Wu PH, Chen YT, Hung JH, Young KC, Lai MD.J Cell Biochem. 2010 Dec 29. [Epub ahead of print]
- Applications
- Western Blot (Cell lysate)
- The ACSL4 polyclonal antibody (Cat # PAB2504) is used in Western blot to detect ACSL4 in RKO cell lysate (human colon cancer) treated with 10% FCS for 24 hrs. Data is kindly provided by Drs. M.G. Sciulli and P. Patrignani from the University of Chieti (Italy).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the ACSL4 polyclonal antibody (Cat # PAB2504) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 2182
- Protein Accession#:
- ACSL4_HUMAN
- Gene Name:
- ACSL4
- Gene Alias:
- ACS4,FACL4,LACS4,MRX63,MRX68
- Gene Description:
- acyl-CoA synthetase long-chain family member 4
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
- Other Designations:
- OTTHUMP00000023846,acyl-CoA synthetase 4,fatty-acid-Coenzyme A ligase, long-chain 4,lignoceroyl-CoA synthase,long-chain fatty-acid-Coenzyme A ligase 4
- Related Disease