Backgound

Interleukin-2 is a protein that has a variety of immunologic functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows an approximately 60% sequence similarity, and human sequence IL-2 has been found to be active on murine cell lines. The 55 kDa IL-2 Rα is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain γc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβ and γc. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rα synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of nave CD4+ T cells but not activated CD4+ memory lymphocytes . IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Source

Recombinant Human IL-2 is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153. Predicted molecular mass 19.2 kDa. This protein carries a polyhistidine tag at the C-terminus.

Recombinant Human IL-2 is expressed from human 293 cells (HEK293)

Endotoxin

Less than 1.0 EU per μg by the LAL method. 

AA Sequence

APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

Purity

Purity >90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human IL-2, His Tag on SDS-PAGE under reducing (R) condition

Human IL-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.