STUB1 polyclonal antibody
产品名称： STUB1 polyclonal antibody
英文名称： STUB1 polyclonal antibody
产品编号： PAB1694
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of STUB1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human STUB1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase.
Peng HM, Morishima Y, Jenkins GJ, Dunbar AY, Lau M, Patterson C, Pratt WB, Osawa Y.J Biol Chem. 2004 Dec 17;279(51):52970-7. Epub 2004 Oct 4.
- 2.
- Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP.Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12130-5. Epub 2004 Aug 9.
- 3.
- The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator.
Alberti S, Bohse K, Arndt V, Schmitz A, Hohfeld J.Mol Biol Cell. 2004 Sep;15(9):4003-10. Epub 2004 Jun 23.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of STUB1 polyclonal antibody (Cat # PAB1694) in T-47D cell lysate. STUB1 (arrow) was detected using the purified polyclonal antibody.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with STUB1 polyclonal antibody (Cat # PAB1694) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 10273
- Protein Accession#:
- Q969U2
- Gene Name:
- STUB1
- Gene Alias:
- CHIP,HSPABP2,NY-CO-7,SDCCAG7,UBOX1
- Gene Description:
- STIP1 homology and U-box containing protein 1
- Omim ID:
- 607207
- Gene Ontology:
- Hyperlink
- Gene Summary:
- STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM
- Other Designations:
- heat shock protein A binding protein 2 (c-terminal),serologically defined colon cancer antigen 7
- Gene Pathway