Camk2a/Camk2b (phospho T286) polyclonal antibody
产品名称： Camk2a/Camk2b (phospho T286) polyclonal antibody
英文名称： Camk2a/Camk2b (phospho T286) polyclonal antibody
产品编号： PAB9668
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Camk2a/Camk2b.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding T286 of rat Camk2a/Camk2b.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 50, 60
- Reactivity:
- Clawed frog, Human, Mouse, Rat
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 1 mg/mL BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats.
Meng F, Guo J, Zhang Q, Song B, Zhang G.Brain Res. 2003 Mar 28;967(1-2):161-9.
- 2.
- Translocation of CaM kinase II to synaptic sites in vivo.
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR.Nat Neurosci. 2003 Mar;6(3):217-8.
- 3.
- Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ.Neuron. 2002 Oct 24;36(3):493-505.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat brain lysate showing specific immunolabeling of the ~50 KDa- and the ~60 KDa Camk2a/Camk2b phosphorylated at Thr286 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Camk2a/Camk2b T286. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
- Entrez GeneID:
- 25400
- Protein Accession#:
- P11275
- Gene Name:
- Camk2a
- Gene Alias:
- PK2CDD,PKCCD
- Gene Description:
- calcium/calmodulin-dependent protein kinase II alpha
- Gene Ontology:
- Hyperlink
- Other Designations:
- Ca2+/calmodulin-dependent protein kinase II alpha,alpha CaM kinase II,calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha,calcium/calmodulin-dependent protein kinase II alpha subunit
- Entrez GeneID:
- 24245
- Protein Accession#:
- P11275
- Gene Name:
- Camk2b
- Gene Alias:
- Ck2b
- Gene Description:
- calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
- Gene Ontology:
- Hyperlink
- Other Designations:
- Ca++/calmodulin-dependent protein kinase II beta subunit,Ca++/calmodulin-dependent protein kinase II, beta subunit,Ca2+/calmodulin-dependent protein kinase II beta,calcium/calmodulin-dependent protein kinase II beta subunit,calcium/calmodulin-dependent pr
- Interactome
