UBE2S polyclonal antibody
产品名称： UBE2S polyclonal antibody
英文名称： UBE2S polyclonal antibody
产品编号： PAB1701
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBE2S.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2S.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.
Garnett MJ, Mansfeld J, Godwin C, Matsusaka T, Wu J, Russell P, Pines J, Venkitaraman AR.Nat Cell Biol. 2009 Nov;11(11):1363-9. Epub 2009 Oct 11.
- Applications
- Western Blot (Cell lysate)
- UBE2S polyclonal antibody (Cat # PAB1701) is used in Western blot to detect UBE2S in Jurkat cell lysate.
- Western Blot (Transfected lysate)
- Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1701). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1701) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 27338
- Protein Accession#:
- NP_055316;Q16763
- Gene Name:
- UBE2S
- Gene Alias:
- E2-EPF,E2EPF,EPF5
- Gene Description:
- ubiquitin-conjugating enzyme E2S
- Omim ID:
- 610309
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq
- Other Designations:
- ubiquitin carrier protein S,ubiquitin-conjugating enzyme E2-24 kD,ubiquitin-protein ligase S
- Gene Pathway