ATG10 polyclonal antibody
产品名称： ATG10 polyclonal antibody
英文名称： ATG10 polyclonal antibody
产品编号： PAB0732
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ATG10.
- Immunogen:
- A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to C-terminus of human ATG10.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Lyophilized
- Storage Buffer:
- Lyophilized from PBS
- Storage Instruction:
- Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Immunohistochemistry (1:100-1:1000)
Immunofluorescence (1:100-1:1000)
The optimal working dilution should be determined by the end user.
- Applications
- Immunofluorescence
- Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody (Cat # PAB0732) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.
- Entrez GeneID:
- 83734
- Protein Accession#:
- Q9H0Y0
- Gene Name:
- ATG10
- Gene Alias:
- APG10,APG10L,DKFZp586I0418,FLJ13954,pp12616
- Gene Description:
- ATG10 autophagy related 10 homolog (S. cerevisiae)
- Omim ID:
- 610800
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
- Other Designations:
- APG10 autophagy 10-like
- Related Disease