HARS2 (N-term) Peptide
产品名称： HARS2 (N-term) Peptide
英文名称： HARS2 (N-term) Peptide
产品编号： P2595
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of HARS2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HARS2.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemsitry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Histidyl-tRNA synthetase.
Freist W, Verhey JF, Ruhlmann A, Gauss DH, Arnez JG.Biol Chem. 1999 Jun;380(6):623-46.
- 2.
- A novel gene oriented in a head-to-head configuration with the human histidyl-tRNA synthetase (HRS) gene encodes an mRNA that predicts a polypeptide homologous to HRS.
O'Hanlon TP, Raben N, Miller FW.Biochem Biophys Res Commun. 1995 May 16;210(2):556-66.
- 3.
- Transcriptional analyses of the gene region that encodes human histidyl-tRNA synthetase: identification of a novel bidirectional regulatory element.
Tsui HW, Mok S, de Souza L, Martin A, Tsui FW.Gene. 1993 Sep 15;131(2):201-8.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of HARS2 polyclonal antibody (Cat # PAB2982) in A2058 cell line lysates (35 ug/lane).HARS2 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human testisreacted with HARS2 polyclonal antibody (Cat # PAB2982), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 23438
- Protein Accession#:
- NP_036340;P49590
- Gene Name:
- HARS2
- Gene Alias:
- HARSL,HARSR,HO3
- Gene Description:
- histidyl-tRNA synthetase 2, mitochondrial (putative)
- Omim ID:
- 600783
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq
- Other Designations:
- HARS-related,hisRS,histidine tRNA ligase 2, mitochondrial (putative),histidine translase,histidine-tRNA ligase homolog,histidyl-tRNA synthetase 2,histidyl-tRNA synthetase-like
- Gene Pathway